Coding
Kozak

Part:BBa_K4574027

Designed by: Igor Koop   Group: iGEM23_Munich   (2023-10-05)


GCCACC Kozak

Vertebrate consensus sequence which enhances the initiation of translation in eukaryotic cells by promoting ribosome recognition and binding to the mRNA's start codon.

Source

Common motif for mammalian recombinant protein expression.

Usage and Biology

This basic part has been used as a component of several antibody constructs in mammalian cell culture (HEK293T, Exi293, RAMOS).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References


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Categories
Parameters
None